The dependence of the ratio of leukocytes in peripheral blood and immunocompetent organs of the bream Abramis brama L., inhabiting the Ivankovskoye Reservoir, on the length, weight, sex and age of the fish was studied. In smears, the cell types characteristic of the species were differentiated: lymphocytes, monocytes/macrophages, neutro- and eosinophils, blast cells. The distribution of individual values of leukocyte forms for each organ was normal according to the chi-square criterion with left-sided (lymphocytes) and right-sided asymmetry (neutrophils, eosinophils and blast cells). The number of leukocyte forms did not correlate with the length, weight and age of the fish. The differences between males and females were most pronounced in the composition of leukocytes in the head kidney: males had a higher content of monocytes, neutrophils and eosinophils, and a lower content of blast cells. In the spleen of females, the relative content of neutrophils and eosinophils is higher than in males.
bream Abramis brama, peripheral blood, pronephros, spleen, leukocytes, Ivankovskoye Reservoir
1. Andreeva A.M. Oogenez lescha Abramis brama (L.) (Cyprinidae) Rybinskogo vodohranilischa // Vopr. ihtiologii. 1983. T. 23. Vyp. 2. S. 257–261.
2. Atlas presnovodnyh ryb Rossii. T. 1. M.: Nauka, 2002. 379 s.
3. Basova M.M. Leykocitarnaya formula morskogo ersha Scorpaena porcus kak biomarker antropogennogo zagryazneniya pribrezhnyh vod Chernogo morya // Vopr. ihtiologii. 2017. T. 57, № 3. S. 347–352. DOI:https://doi.org/10.7868/S004287521703002X.
4. Brehovskih V.F., Kazmiruk T.N., Kazmiruk V.D. Donnye otlozheniya Ivan'kovskogo vodohranilischa: sostoyanie, sostav, svoystva. M.: Nauka, 2006. 176 s.
5. Golovina N.A., Trombickiy I.D. Gematologiya prudovyh ryb. Kishinev: Shtiinca, 1989. 155 s.
6. Gordeev I.I., Balabanova L.V., Suvorova T.A. i dr. Sostav leykocitov perifericheskoy krovi i immunokompetentnyh organov belogo gol'ca ozera Kronockoe (Kamchatka) // Rybovodstvo i rybnoe hozyaystvo. 2021. № 9(188). S. 42–49. DOI:https://doi.org/10.33920/sel-09-2109-04.
7. Goryachev D.V., Nikitenko A.I., Klec N.N. i dr. Sostoyanie zapasov vodnyh biologicheskih resursov Ivan'kovskogo i Uglichskogo vodohranilisch // Vopr. rybolovstva. 2021. T. 22, № 1. S. 25–37. DOI:https://doi.org/10.36038/0234-2774-2021-22-1-25-37.
8. Grigor'eva I.L., Komissarov A.B., Chekmareva E.A. Sovremennoe sostoyanie, istochniki zagryazneniya i vozmozhnye puti reabilitacii Ivan'kovskogo vodohranilischa i ego pritokov // Nauchnye problemy ozdorovleniya rossiyskih rek i puti ih resheniya: Sbornik nauchnyh trudov, Nizhniy Novgorod, 08–14 sentyabrya 2019 g. Nizhniy Novgorod: Studiya F1, 2019. S. 332–336.
9. Zhiteneva L.D., Poltavceva T.G., Rudnickaya O.A. Atlas normal'nyh i patologicheski izmenennyh kletok krovi ryb: spravochnik. Rostov-na-Donu: Rostovskoe knizhnoe izdatel'stvo, 1989. 112 s.
10. Zhiteneva T.S. Osobennosti ekologii lescha Abramis brama L. na etapah i perehodnyh periodah razvitiya v svyazi s problemoy ego rosta v Ivan'kovskom vodohranilische // Biol. vnutr. vod. 1998. № 1. S. 55–61.
11. Ivanova N.T. Atlas kletok krovi ryb. M.: Leg. i pisch. prom-st', 1983. 184 s.
12. Ivan'kovskoe vodohranilische i ego zhizn' (pod red. N.V. Butorina). L.: Nauka, 1978. 304 s.
13. Keyster I.A. Dinamika pokazateley krovi ryb kak pokazatel' sostoyaniya ihtiofauny Belogo ozera (Vologodskaya oblast') // Zhurn. nauch. publikaciy aspirantov i doktorantov. 2007. № 12 (http://jurnal.org/articles/2007/bio6.html.Version 03/2022).
14. Kovaleva M.P. Tehnika promyshlennogo rybolovstva na Ivan'kovskom vodohranilische // Izv. GosNIORH. 1964. T. 56. S. 42–70.
15. Korneva L.G., Solov'eva V.V., Makarova O.S. i dr. Raspredelenie planktona v volzhskih vodohranilischah letom 2015 g. // Trudy Instituta biologii vnutrennih vod im. I.D. Papanina RAN. 2018. Vyp. 82(85). S. 21–27. DOI:https://doi.org/10.24411/0320-3557-2018-1-0010.
16. Kuzina T.V. Citofiziologicheskie osobennosti krovi promyslovyh ryb Volgo-Kaspiyskogo kanala. Avtoref. diss. … kand. biol. nauk. Astrahan', 2011. 25 s.
17. Lapirova T.B., Zabotkina E.A. Sravnitel'nyy analiz pokazateley immunofiziologicheskogo sostoyaniya lescha Abramis brama (L.) iz razlichnyh po stepeni zagryazneniya uchastkov Rybinskogo vodohranilischa // Biol. vnutr. vod. 2010. № 2. S. 86–91. DOI:https://doi.org/10.1134/S1995082910020136.
18. Lapirova T.B., Flerova E.A. Fiziologo-biohimicheskaya harakteristika krovi lescha (Abramis brama L.) Rybinskogo vodohranilischa // Vestnik Michurinskogo GAU. 2015. № 2. S. 83–88.
19. Mikryakov V.R., Balabanova L.V. Osnovy kletochnogo immuniteta u ryb // Fiziologiya i parazitologiya presnovodnyh zhivotnyh. L.: Nauka, 1979. S. 105–124.
20. Mikryakov V.R., Balabanova L.V., Zabotkina E.A. i dr. Reakciya immunnoy sistemy ryb na zagryaznenie vody toksikantami i zakislenie sredy. M.: Nauka, 2001. 126 s.
21. Mikryakov V.R., Lapirova T.B. Vliyanie soley nekotoryh tyazhelyh metallov na sostav beloy krovi molodi lenskogo osetra Acipenser baeri // Vopr. ihtiologii. 1997. T. 37, № 4. S. 538–542.
22. Mikryakov V.R., Tereschenko V.G., Mikryakov D.V. Opyt primeneniya integral'nogo indeksa dlya ocenki destabilizacionnyh processov v immunnoy sisteme ryb // Biol. vnutr. vod. 2021. № 3. S. 311–320. DOI:https://doi.org/10.31857/S0320965221030104.
23. Mineeva N.M., Semadeni I.V., Solov'eva V.V., Makarova O.S. Soderzhanie hlorofilla i sovremennoe troficheskoe sostoyanie vodohranilisch r. Volgi (2019–2020 gg.) // Biol. vnutr. vod. 2022. № 4. S. 367–371. DOI:https://doi.org/10.31857/S0320965222040210.
24. Nikanorov Yu.I. Ivan'kovskoe i Uglichskoe vodohranilische // Sb. nauch. tr. GosNIORH. 1984. Vyp. 210. S. 4–12.
25. Perova S.N., Pryanichnikova E.G., Zhgareva N.N., Zubishina A.A. Taksonomicheskiy sostav i obilie makrozoobentosa volzhskih vodohranilisch // Trudy Instituta biologii vnutrennih vod im. I.D. Papanina RAN. 2018. Vyp. 82(85). S. 52–66. DOI:https://doi.org/10.24411/0320-3557-2018-1-0012.
26. Petrov R.V. Immunologiya. M.: Medicina, 1987. 416 s.
27. Savina L.V. Ispol'zovanie gematologicheskih pokazateley ryb dlya ocenki novoy kormovoy dobavki MIK BAK i ekologicheskogo sostoyaniya estestvennyh vodoemov. Avtoref. diss. … kand. biol. nauk. Kaliningrad, 2004. 24 s.
28. Sakun O.F., Buckaya N.A. Opredelenie stadiy zrelosti i izuchenie polovyh ciklov ryb. M.: Rybnoe hozyaystvo, 1963. 35 s.
29. Sappo G.B. Biologiya, zapasy lescha Ivan'kovskogo vodohranilischa i vliyanie na nih sbrosnyh vod Konakovskoy GRES. Avtoref. diss. ... kand. biol. nauk. Leningrad, 1976. 25 s.
30. Svetasheva D.R., Grushko M.P., Nguen T.H.V. Razvitie i funkcional'naya organizaciya central'nyh organov gemopoeza lichinok lescha obyknovennogo (Abramis brama (L.)) // Vestnik Astrahanskogo gosudarstvennogo tehnicheskogo universiteta. Seriya: Rybnoe hozyaystvo. 2019. № 3. S. 125–131. DOI:https://doi.org/10.24143/2073-5529-2019-3-125-131.
31. Serpunin G.G. Gematologicheskie pokazateli adaptaciy ryb. Avtoref. dis. ... dokt. biol. nauk. Kaliningrad, 2002. 49 s.
32. Suvorova T.A., Pronina G.I., Mikryakov D.V. Sostav leykocitov perifericheskoy krovi i immunokompetentnyh organov krossa karpa “Petrovskiy” v raznye periody rybovodnogo sezona // Rybovodstvo i ryb. hoz-vo. 2021. № 1. S. 58–68. DOI:https://doi.org/10.33920/sel-09-2101-05.
33. Haitov R.M., Ignat'eva G.A., Sidorovich I.G. Immunologiya: Uchebnik. M.: Medicina, 2002. 536 s.
34. Ekologicheskie problemy Verhney Volgi (pod red. Kopylova A.I.). Yaroslavl': YaGTU, 2001. 427 s.
35. Yurova O.V., Sudarev N.P. Prirodnoe i antropogennoe vliyanie na populyaciyu lescha v Ivan'kovskom vodohranilische // Agrarnyy vestnik Verhnevolzh'ya. 2022. № 2(39). S. 79–85. DOI:https://doi.org/10.35523/2307-5872-2022-39-2-79-85.
36. Ellis A.E. Ontogeny of the Immune System in Teleost Fish // Fish Vaccination. 1988. P. 20–31.
37. Fänge R., Nilsson S. The fish spleen: structure and function // Experimentia. 1985. Vol. 41, № 2. P. 152–158.
38. Fazio F., Lanteri G., Saoca C. et al. Individual variability of blood parameters in striped bass Morones axatilis: possible differences related to weight and length // Aquaculture International. 2020. Vol. 28. P. 1665–1673. DOI:https://doi.org/10.1007/s10499-020-00550-z.
39. Hamdani S.H., McMillan D.N., Pettersen E.F. et al. Isolation of rainbow trout neutrophils with an anti-granulocyte monoclonal antibody // Vet. Immunol. Immunopathol. 1998. Vol. 63. P. 369–380.
40. Havixbeck J.J., Rieger A.M., Wong M.E. et al. Neutrophil contributions to the induction and regulation of the acute inflammatory response in teleost fish // J. Leukoc. Biol. 2016. Vol. 99, № 2. P. 241–252. DOI:https://doi.org/10.1189/jlb.3HI0215-064R.
41. Karim B., Yousria G., Wyllia Kh. Influence of total length, sex and seasonal variations on hematological parameters in Cyprinus carpio (Linnaeus, 1758) (Pisces Cyprinidae) in Lake Tonga (Algeria) // Biodivers. J. 2019. Vol. 10(4). P. 593–600. DOI:https://doi.org/10.31396/Biodiv.Jour.2019.10.4.593.600.
42. Katzenback B.A., Belosevic M. Isolation and functional characterization of neutrophil-like cells, from goldfish (Carassius auratus L.) kidney // Dev. Comp. Immunol. 2009. Vol. 33, № 4. P. 601–611. DOI:https://doi.org/10.1016/j.dci.2008.10.011.
43. Kostić‑Vuković J., Kolarević S., Kračun‑Kolarević·M. et al. Temporal variation of biomarkers in common bream Abramis brama (L., 1758) exposed to untreated municipalwastewater in the Danube River in Belgrade, Serbia // Environ. Monit. Assess. 2021. Vol. 193(8). P. 465. DOI:https://doi.org/10.1007/s10661-021-09232-6.
44. Križanac S., Topi´cPopovi´c N., Bariši´c J. et al. Comparative Study of Physiological Changes in Turbot Scophthalmus maximus in Different Living Conditions // Appl. Sci. 2022. Vol. 12(9). P. 4201. DOI:https://doi.org/10.3390/app12094201.
45. Lamkova K., Simkova A., Palikova M. et al. Seasonal changes of immunocompetence and parasitism in chub (Leuciscus cephalus), a freshwater cyprinid fish // Parasitol. Res. 2007. Vol. 101. P. 775–789. DOI:https://doi.org/10.1007/s00436-007-0546-3.
46. Lapirova T.B., Zabotkina E.A. Effect of trypanosomiasis on hematologic characteristics of bream (Abramis brama) // Regul. Mech. Biosyst. 2018. Vol. 8(3). P. 309–314. DOI:https://doi.org/10.15421/021845.
47. Modra H., Svobodova Z., Kolafova I. Comparison of Differential Leukocyte Counts in Fish of Economic and Indicator Importance // Acta Vet. Brno. 1998. Vol. 67. P. 215–226. DOI:https://doi.org/10.2754/avb199867040215.
48. Noga E.J. Spleen, thymus, reticulo-endothelial system, blood // Systemic pathology of fish. A text and atlas of normal tissues in teleosts and their responses in disease. London: Scotian Press. 2006. 121 p.
49. Parish N., Wrathmell A., Hart S., Harris J. The leucocytes of the elasmobranch Scyliorhinus vanicula L. A morphological study // J. Fish. Biol. 1986. Vol. 28, № 5. P. 545–561. DOI:https://doi.org/10.1111/j.1095-8649.1986.tb05192.x.
50. Pavlidis M., Futter W.C., Kathario P., Divanach P. Blood cells of six Mediterranean mariculture fish species // J. Appl. Ichthyology. 2007. Vol. 23. P. 70–73. DOI:https://doi.org/10.1111/j.1439-0426.2006.00771x.
51. Rey Vazquez G., Guerrero G.A. Characterization of blood cells and hematological parameters in Cichlasoma dimerus (Teleostei, Perciformes) // Tissue Cell. 2007. Vol. 39. P. 151–160. DOI:https://doi.org/10.1016/j.tice.2007.02.004.
52. Samai H.C., Rioult D., Bado-Nilles A. et al. Procedures for leukocytes isolation from lymphoid tissues and consequences on immune endpointsused to evaluate fish immune status: A case study on roach (Rutilus rutilus) // Fish and Shellfish Immunol. 2018. Vol. 74. P. 643–657. DOI:https://doi.org/10.1016/j.fsi.2017.12.040.
53. Sasaki Y., Maita M., Okamoto N. Rainbow trout neutrophils are responsible for non-specific cytotoxicity // Fish Shellfish Immunol. 2002. Vol. 14. P. 243–252. DOI:https://doi.org/10.3390/biology4040715.
54. Scapigliati G. Functional aspects of fish lymphocytes // Dev. Comp. Immunol. 2013. Vol. 41, № 2. P. 200–208. DOI:https://doi.org/10.1016/j.dci.2013.05.012.
55. Smith C.R., Ottinger C.A., Walsh H.L., Blazer V.S. Development of a suite of functional immune assays and initial assessment of their utility in wild smallmouth bass health assessments. Reston: U.S. Geological Survey, 2020. Open-File Report 2020–1077. 23 p. DOI:https://doi.org/10.3133/ofr20201077.
56. Sueiro M.C., Awruch C., Gilardoni C. et al. Immunity and health of two wild marine fishes naturally exposed to anthropogenic pollution // Sci. Total Env. 2020. Vol. 726. P. 138–303. DOI:https://doi.org/10.1016/j.scitotenv.2020.138303.
57. Suvorova T.A., German A.V. Percentage of Leukocytes in Peripheral Blood, Head Kidney, and Spleen of the Abramis brama (Cyprinidae) Bream of the Upper Volga Reservoirs // J. Ichthyol. 2024. Vol. 64, № 2. P. 352–356. DOI:https://doi.org/10.1134/S0032945224020140.
58. Tenji D., Micic B., Sipos S. et al. Fish biomarkers from a different perspective: evidence of adaptive strategy of Abramis brama (L.) to chemical stress // Environ. Sci. Eur. 2020. Vol. 32, № 1. P. 38. DOI:https://doi.org/10.1186/s12302-020-00316-7.
59. Uribe C., Folch H., Enriquez R., Moran G. Innate and adaptive immunity in teleost fish: A review // Vet. Med. 2011. Vol. 56, № 10. P. 486–503. DOI:https://doi.org/10.17221/3294-VETMED.
60. Vainikka A., Jokinen E.I., Kortet R., Taskinen J. Gender- and season-dependent relationships between testosterone, oestradiol and immune function in wild roach // J. Fish Biol. 2004. Vol. 64. P. 227–240. DOI:https://doi.org/10.1046/j.1095-8649.2004.00306.x.
61. Yadav D.P., Banaijee V., Banaerjee M. Haematology of genus Channa: leucocytes // Comp. Physiol. Ecol. 1986. Vol. 11, № 4. P. 226–232.
62. Zapata A., Amemiya C.T. Phylogeny of Lower Vertebrates and Their Immunological Structures // Current Topics in Microbiology and Immunology Microbiology. 2000. Vol. 248. P. 67–107. DOI:https://doi.org/10.1007/978-3-642-59674-2_5.
63. Zapata A.G. Ultrastructural study of the teleost fish kidney // Develop. Comp. Immunol. 1979. №. 3. P. 55–65.
